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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human Cyclophilin F
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Gene ID
10105
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Swiss Prot
P30405
- Synonyms CYP3; CypD; CyP-M; Cyp-D
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000
IHC-P: 1:500-1:5000
ICC/IF: 1:100
FC: 1:200-1:500 - Calculated MW 22 kDa
- Observed MW 18 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15454
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, 3T3, HeLa cells using db11820 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11820 antibody. -
Western blot analysis of extracts from Rat heart tissue using db11820 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Cyclophilin F with db11820.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11820 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
