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RBBP4 (DGR16021) Rabbit mAb (PBS Only)
db11829-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
- Immunogen A synthetic peptide of human RbAp48
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Gene ID
5928
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Swiss Prot
Q09028
- Synonyms NURF55; RBAP48; lin-53
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 48 kDa
- Observed MW 48 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16021
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, CHO-K1, C6, Jurkat cells using db11829 at 1:500. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db11829 antibody. -
Western blot analysis of extracts from Mouse spleen tissue using db11829 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling RBBP4 with db11829.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11829 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
