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Caspase-3 (DGR13170) Rabbit mAb (PBS Only)
db12058-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
- Immunogen A synthetic peptide of human Caspase-3
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Gene ID
836
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Swiss Prot
P42574
- Synonyms CPP32; SCA-1; CPP32B
- Reactivity Human
- Application WB, IHC, ICC/IF, FC, IP
- Calculated MW 32 kDa
- Observed MW 32,17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13170
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, HeLa cells using db12058 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using db12058 antibody. -
Immunofluorescent analysis of 293 cells using db12058 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling Caspase-3 with db12058.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12058 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1498
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
