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Tuberin (DGR32325) Rabbit mAb (PBS Only)
db12165-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human Tuberin
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Gene ID
7249
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Swiss Prot
P49815
- Synonyms LAM; TSC4; PPP1R160
- Reactivity Human
- Application WB, IHC, ICC/IF, FC, IP
- Calculated MW 201 kDa
- Observed MW 201 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR32325
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from 293T cells using db12165 at 1:1000. -
Western blot analysis of extracts from A431 cells using db12165 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human hepatic carcinoma using db12165 antibody. -
Immunofluorescence analysis of HeLa cells labelling Tuberin with db12165.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12165 (1:20) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
