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Cyclin A2 (DGR14328) Rabbit mAb (PBS Only)
db12205-PBS
Datasheet

Product Name : Cyclin A2 (DGR14328) Rabbit mAb (PBS Only)

Cat.No.: db12205-PBS

Synonyms : CCN1; CCNA

Application:

Reactivity : Human, Mouse

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]
  • Immunogen A synthetic peptide of human Cyclin A2
  • Gene ID 890
  • Swiss Prot P20248
  • Synonyms CCN1; CCNA
  • Reactivity Human,Mouse
  • Application WB, IHC-P, ICC/IF, IP
  • Calculated MW 49 kDa
  • Observed MW 49 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR14328
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from K562, C6, 3T3, HeLa cells using db12205 at 1:1000.
  • Immunohistochemical analysis of paraffin-embedded human tonsil using db12205 antibody.
  • Immunofluorescent analysis of HeLa cells using db12205 antibody (green), and DAPI (blue).
  • Immunofluorescence analysis of HeLa cells labelling Cyclin A2 with db12205.

    The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12205 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 1898
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.