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DROSHA (DGR33325) Rabbit mAb (PBS Only)
db12329-PBS
Datasheet
Buffer&Conjugation
- Background This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
- Immunogen Recombinant protein of human DROSHA
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Gene ID
29102
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Swiss Prot
Q9NRR4
- Synonyms RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC
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Recommended dilution
WB: 1:1000
ICC/IF: 1:50
FC: 1:200-1:500 - Calculated MW 159 kDa
- Observed MW 159 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR33325
- Isotype IgG
- Purity Affinity Purification
- Conjugation PBS Only
- Concentration 1mg/mL
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling DROSHA with db12329.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12329 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
