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Conjugation/Formulation：Un-conjugated
- Background This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]
- Immunogen Recombinant protein of human DGAT1
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Gene ID
8694
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Swiss Prot
O75907
- Synonyms ARAT; DGAT; ARGP1; DIAR7
- Reactivity Human
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:2000-1:20000
ICC/IF: 1:50-1:100
FC: 1:200-1:500
IP: 1:20-1:50 - Calculated MW 55 kDa
- Observed MW 55 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16581
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562 cells using db12413 at 1:1000. -
Western blot analysis of extracts from T24 cells using db12413 at 1:5000. -
Immunofluorescence analysis of MCF-7 cells labelling DGAT1 with db12413.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12413 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
