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beta Tubulin (DGR21047) Rabbit mAb (PBS Only)
db12470-PBS
Datasheet

Product Name : beta Tubulin (DGR21047) Rabbit mAb (PBS Only)

Cat.No.: db12470-PBS

Synonyms : M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
  • Immunogen A synthetic peptide of human beta Tubulin
  • Gene ID 203068
  • Swiss Prot P07437
  • Synonyms M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
  • Reactivity Human,Mouse,Rat
  • Application WB, IHC-P, ICC/IF, FC
  • Calculated MW 50 kDa
  • Observed MW 50 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR21047
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from HeLa, Jurkat, HepG2, K562, C6 cells and Rat brain tissue using db12470 at 1:5000.
  • Western blot analysis of extracts from 3T3, RAW264.7, PC-12 cells and Rat lung, Mouse lung tissues using db12470 at 1:5000.
  • Western blot analysis of extracts from Mouse brain tissue using db12470 at 1:5000.
  • Immunofluorescence analysis of HeLa cells labelling beta Tubulin with db12470.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12470 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 2598
  • 100μg 7858
  • 货期:现货
For research use only. Not intended for diagnostic.