beta Tubulin (DGR21047) Rabbit mAb (PBS Only)
Product Name :
beta Tubulin (DGR21047) Rabbit mAb (PBS Only)
Cat.No.:
db12470-PBS
Synonyms :
M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Reactivity :
Human, Mouse, Rat
Host species :
Rabbit
Conjugation/Formulation:PBS Only
-
Background
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
-
Immunogen
A synthetic peptide of human beta Tubulin
-
Gene ID
203068
-
Swiss Prot
P07437
-
Synonyms
M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
-
Reactivity
Human,Mouse,Rat
-
Application
WB, IHC-P, ICC/IF, FC
-
Calculated MW
50 kDa
-
Observed MW
50 kDa
-
Host species
Rabbit
-
Clonality
Monoclonal
-
Clonality No.
DGR21047
-
Isotype
IgG
-
Purity
Affinity Purification
-
Conjugation
Un-conjugated
-
Concentration
1 mg/mL
-
Formulation
PBS Only
-
Storage/Stability
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
-
-
Western blot analysis of extracts from HeLa, Jurkat, HepG2, K562, C6 cells and Rat brain tissue using
db12470 at 1:5000.
-
Western blot analysis of extracts from 3T3, RAW264.7, PC-12 cells and Rat lung, Mouse lung tissues using
db12470 at 1:5000.
-
Western blot analysis of extracts from Mouse brain tissue using
db12470 at 1:5000.
-
Immunofluorescence analysis of HeLa cells labelling beta Tubulin with
db12470.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with
db12470 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
-
Package
Price (RMB)
-
10μg
2598
-
100μg
7858
- 货期:现货
For research use only. Not intended for diagnostic.