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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
- Immunogen A synthetic peptide of human beta Tubulin
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Gene ID
203068
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Swiss Prot
P07437
- Synonyms M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:50
ICC/IF: 1:200-1:500
FC: 1:20 - Calculated MW 50 kDa
- Observed MW 50 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR21047
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, Jurkat, HepG2, K562, C6 cells and Rat brain tissue using db12470 at 1:5000. -
Western blot analysis of extracts from 3T3, RAW264.7, PC-12 cells and Rat lung, Mouse lung tissues using db12470 at 1:5000. -
Western blot analysis of extracts from Mouse brain tissue using db12470 at 1:5000. -
Immunofluorescence analysis of HeLa cells labelling beta Tubulin with db12470.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12470 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 100μL 998
- 500μL 3998
- 1mL 6998
- 货期：现货
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For research use only. Not intended for diagnostic.
