Extracellular matrix protein 1单克隆抗体(DGR17048) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Extracellular matrix protein 1 (DGR17048) Rabbit mAb

db12475

618特价

Datasheet

Product Name : Extracellular matrix protein 1 (DGR17048) Rabbit mAb

Cat.No.: db12475

Synonyms : URBWD

Application：

Reactivity : Human, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
Immunogen A synthetic peptide of human Extracellular matrix protein 1
Gene ID 1893
Swiss Prot Q16610
Synonyms URBWD
Reactivity Human,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000-1:5000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
FC: 1:20-1:50
IP: 1:10-1:100
Calculated MW 61 kDa
Observed MW 61 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR17048
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot analysis of extracts from HeLa cells using db12475 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human tonsil using db12475 antibody.
Immunofluorescent analysis of K562 cells using db12475 antibody (green), and DAPI (blue).
Immunofluorescence analysis of HeLa cells labelling Extracellular matrix protein 1 with db12475.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12475 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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