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Integrin linked ILK (DGR12955) Rabbit mAb (PBS Only)
db12535-PBS
Datasheet

Product Name : Integrin linked ILK (DGR12955) Rabbit mAb (PBS Only)

Cat.No.: db12535-PBS

Synonyms : P59; ILK-1; ILK-2; p59ILK; HEL-S-28

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  • Immunogen A synthetic peptide of human Integrin linked ILK
  • Gene ID 3611
  • Swiss Prot Q13418
  • Synonyms P59; ILK-1; ILK-2; p59ILK; HEL-S-28
  • Reactivity Human,Mouse,Rat
  • Application WB, ICC/IF, FC
  • Calculated MW 51 kDa
  • Observed MW 51 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR12955
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from HeLa cells using db12535 at 1:1000.
  • Immunofluorescence analysis of HeLa cells labelling Integrin linked ILK with db12535.

    The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12535 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 2598
  • 100μg 7858
  • 货期:现货
For research use only. Not intended for diagnostic.