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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
- Immunogen A synthetic peptide of human Integrin linked ILK
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Gene ID
3611
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Swiss Prot
Q13418
- Synonyms P59; ILK-1; ILK-2; p59ILK; HEL-S-28
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC
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Recommended dilution
WB: 1:2000-1:20000
ICC/IF: 1:200-1:500
FC: 1:100 - Calculated MW 51 kDa
- Observed MW 51 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12955
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db12535 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Integrin linked ILK with db12535.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12535 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
