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AKT1/3 (DGR12893) Rabbit mAb (PBS Only)
db12536-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
- Immunogen A synthetic peptide of human AKT1
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Gene ID
207
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Swiss Prot
P31749
- Synonyms AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, IP
- Calculated MW 56 kDa
- Observed MW 56 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12893
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from 3T3 cells using db12536 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling AKT1/3 with db12536.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12536 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
