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Cyclin B1 (DGR14937) Rabbit mAb (PBS Only)
db13216-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Essential for the control of the cell cycle at the G2/M (mitosis) transition.
- Immunogen Recombinant protein of mouse Cyclin B1
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Gene ID
268697
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Swiss Prot
P24860
- Synonyms CycB1; Cycb-4; Cycb-5; Ccnb1-rs1; Cycb1-rs1; Ccnb1-rs13
- Reactivity Human,Mouse
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 48 kDa
- Observed MW 55 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14937
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from A549 cells using db13216 at 1:1000. -
Western blot analysis of extracts from Jurkat cells using db13216 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Cyclin B1 with db13216.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13216 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1498
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
