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Conjugation/Formulation：Un-conjugated
- Background BORA is an activator of the protein kinase Aurora A (AURKA; MIM 603072), which is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis (Hutterer et al., 2006 [PubMed 16890155]).[supplied by OMIM, Mar 2008]
- Immunogen Recombinant protein of human Bora
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Gene ID
79866
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Swiss Prot
Q6PGQ7
- Synonyms C13orf34
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
ICC/IF: 1:100-1:200
IP: 1:20-1:50 - Calculated MW 61 kDa
- Observed MW 61 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR20121
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562,C6 cells using db13303 at 1:1000. -
Western blot analysis of extracts from Mouse liver tissue using db13303 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Bora with db13303.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13303 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
