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Conjugation/Formulation：Un-conjugated
- Background This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- Immunogen A synthetic peptide of human Lamin B1
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Gene ID
4001
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Swiss Prot
P20700
- Synonyms LMN; ADLD; LMN2; LMNB
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC: 1:200
ICC/IF: 1:200
IP: 1:20 - Calculated MW 66 kDa
- Observed MW 66 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13868
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, 3T3, HeLa cells using db13332 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Lamin B1 with db13332.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13332 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
