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NF-kB p65 (DGR19469) Rabbit mAb (PBS Only)
db13524-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
- Immunogen A synthetic peptide of human NF-kB p65
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Gene ID
5970
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Swiss Prot
Q04206
- Synonyms p65; NFKB3
- Reactivity Human, Mouse, Rat
- Application WB, ICC/IF, FC
- Calculated MW 60 kDa
- Observed MW 65 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19469
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat, C6 cells using db13524 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling NF-KB p65 with db13524.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13524 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
