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Histone H2A.X (DGR20837) Rabbit mAb (PBS Only)
db13705-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
- Immunogen A synthetic peptide of human Histone H2A.X
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Gene ID
3014
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Swiss Prot
P16104
- Synonyms H2A.X; H2A/X; H2AFX
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 15 kDa
- Observed MW 15 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR20837
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa and Ramos cells using db13705 at 1:1000. -
Western blot analysis of extracts from Jurkat cells using db13705 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using db13705 antibody. -
Immunofluorescence analysis of HeLa cells labelling Histone H2A.X with db13705.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13705 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
