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CDC42 (DGR15342) Rabbit mAb (PBS Only)
db14118-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
- Immunogen Recombinant protein of human CDC42
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Gene ID
998
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Swiss Prot
P60953
- Synonyms TKS; G25K; CDC42Hs
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 21 kDa
- Observed MW 21 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15342
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, HeLa cells using db14118 at 1:1000. -
Immunofluorescence analysis of MCF-7 cells labelling CDC42 with db14118.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14118 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
