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Conjugation/Formulation：Un-conjugated
- Background This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]
- Immunogen A synthetic peptide of human Cytokeratin 17
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Gene ID
3872
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Swiss Prot
Q04695
- Synonyms PC; K17; PC2; 39.1; CK-17; PCHC1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
FC: 1:20-1:50
IP: 1:20 - Calculated MW 48 kDa
- Observed MW 48 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16018
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db14214 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using db14214 antibody. -
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 17 with db14214.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14214 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
