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ELK1 (DGR19915) Rabbit mAb (PBS Only)
db14344-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]
- Immunogen A synthetic peptide of human ELK1
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Gene ID
2002
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Swiss Prot
P19419
- Synonyms ETS domain-containing protein Elk-1
- Reactivity Human
- Application WB, ICC/IF
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100 - Calculated MW 45 kDa
- Observed MW 62 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19915
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/ml
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, 3T3, HeLa cells using db14344 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling ELK1 with db14344.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14344 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
