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c-Jun (DGR19004) Rabbit mAb (PBS Only)
db14460-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human c-Jun
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Gene ID
3725
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Swiss Prot
P05412
- Synonyms AP1; p39; AP-1; c-Jun
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 36 kDa
- Observed MW 48,43 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19004
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HepG2, A431 cells using db14460 at 1:1000. -
Immunofluorescent analysis of 293T cells using db14460 antibody (green), and DAPI (blue). -
Western blot analysis of extracts from 293, 293T cells using db14460 at 1:1000. -
Western blot analysis of extracts from 3T3 cells using db14460 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling c-Jun with db14460.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14460 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 2598
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
