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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human GRSF1
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Gene ID
2926
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Swiss Prot
Q12849
- Synonyms G-rich sequence factor 1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:100
FC: 1:100-1:200 - Calculated MW 53 kDa
- Observed MW 53 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR31531
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot detection of GRSF1 in Hela,Ramos cell lysates using GRSF1 antibody(1:1000 diluted). -
Immunofluorescent analysis of CEM cells using db14889 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling GRSF1 with db14889.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14889 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
