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Fibronectin (DGR15429) Rabbit mAb (PBS Only)
db15058-PBS
Datasheet

Product Name : Fibronectin (DGR15429) Rabbit mAb (PBS Only)

Cat.No.: db15058-PBS

Synonyms : Fn; Fn-1; E330027I09

Application:

Reactivity : Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
  • Immunogen Recombinant protein of mouse Fibronectin
  • Gene ID 14268
  • Swiss Prot P11276
  • Synonyms Fn; Fn-1; E330027I09
  • Reactivity Mouse,Rat
  • Application WB, IHC-P, ICC/IF, FC, IP
  • Calculated MW 273 kDa
  • Observed MW 300, 273 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR15429
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from Mouse whole blood tissue using db15058 at 1:1000.
  • Immunofluorescence analysis of 3T3 cells labelling Fibronectin with db15058.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15058 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 1898
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.