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IRF2 (DGR15085) Rabbit mAb (PBS Only)
db15148-PBS
Datasheet

Product Name : IRF2 (DGR15085) Rabbit mAb (PBS Only)

Cat.No.: db15148-PBS

Synonyms : IRF-2

Application:

Reactivity : Human, Mouse

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008]
  • Immunogen A synthetic peptide of human IRF2
  • Gene ID 3660
  • Swiss Prot P14316
  • Synonyms IRF-2
  • Reactivity Human,Mouse
  • Application WB, IHC-P, ICC/IF
  • Recommended dilution WB: 1:1000
    IHC-P: 1:50
    ICC/IF: 1:200-1:500
  • Calculated MW 39 kDa
  • Observed MW 45 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR15085
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/ml
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Immunofluorescence analysis of HeLa cells labelling IRF2 with db15148.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15148 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
  • Western blot analysis of extracts from RAW264.7 cells using db15148
订购信息
  • Package Price (RMB)
  • 100μg 4498
  • 货期:现货
For research use only. Not intended for diagnostic.