IRF2 (DGR15085) Rabbit mAb (PBS Only)
Product Name :
IRF2 (DGR15085) Rabbit mAb (PBS Only)
Cat.No.:
db15148-PBS
Synonyms :
IRF-2
Reactivity :
Human, Mouse
Host species :
Rabbit
Conjugation/Formulation:PBS Only
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Background
IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008]
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Immunogen
A synthetic peptide of human IRF2
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Gene ID
3660
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Swiss Prot
P14316
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Synonyms
IRF-2
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Reactivity
Human,Mouse
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Application
WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000
IHC-P: 1:50
ICC/IF: 1:200-1:500
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Calculated MW
39 kDa
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Observed MW
45 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Clonality No.
DGR15085
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Concentration
1 mg/ml
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Formulation
PBS Only
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Storage/Stability
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
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Immunofluorescence analysis of HeLa cells labelling IRF2 with db15148.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15148 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
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Western blot analysis of extracts from RAW264.7 cells using
db15148
订购信息
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Package
Price (RMB)
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100μg
4498
- 货期:现货
For research use only. Not intended for diagnostic.