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CENPC (DGR14946) Rabbit mAb

Product Name : CENPC (DGR14946) Rabbit mAb

Cat.No.: db15194

Synonyms : MIF2; hcp-4; CENP-C; CENPC1

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:Un-conjugated

  • Background Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq, Jul 2008]
  • Immunogen Recombinant protein of human CENPC
  • Gene ID 1060
  • Swiss Prot Q03188
  • Synonyms MIF2; hcp-4; CENP-C; CENPC1
  • Reactivity Human,Mouse,Rat
  • Application WB, IHC-P, ICC/IF, FC, IP
  • Recommended dilution WB: 1:1000-1:5000
    IHC-P: 1:200-1:1000
    ICC/IF: 1:100-1:500
    FC: 1:200-1:500
    IP: 1:100
  • Calculated MW 31 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR14946
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
  • Immunofluorescence analysis of HeLa cells labelling CENPC with db15194.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15194 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μL 398
  • 20μL 698
  • 50μL 1398
  • 100μL 2398
  • 货期:现货
For research use only. Not intended for diagnostic.