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alpha 1 Fetoprotein (DGR14411) Rabbit mAb (PBS Only)
db15311-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Binds estrogens, fatty acids and metals.
- Immunogen Recombinant protein of mouse alpha 1 Fetoprotein
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Gene ID
11576
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Swiss Prot
P02772
- Synonyms Afp
- Reactivity Mouse,Rat
- Application WB, ICC/IF, FC
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100
FC: 1:200-1:500 - Calculated MW 67 kDa
- Observed MW 70 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14411
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/ml
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HepG2 cells labelling alpha 1 Fetoprotein with db15311.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15311 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
