您当前的位置：
Niemann Pick C1 (DGR14389) Rabbit mAb (PBS Only)
db15316-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009]
- Immunogen A synthetic peptide of human Niemann Pick C1
-
Gene ID
4864
-
Swiss Prot
O15118
- Synonyms NPC
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
-
Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:50-1:100
ICC/IF: 1:50-1:100
FC: 1:100-1:200 - Calculated MW 142 kDa
- Observed MW 160-180 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14389
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/ml
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Immunofluorescence analysis of Neuro-2a cells labelling Niemann Pick C1 with db15316.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15316 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
