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Aconitase 1 (DGR14355) Rabbit mAb (PBS Only)
db15331-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
- Immunogen A synthetic peptide of human Aconitase 1
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Gene ID
48
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Swiss Prot
P21399
- Synonyms IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC
- Calculated MW 98 kDa
- Observed MW 98 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14355
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling Aconitase 1 with db15331.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15331 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1498
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
