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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]
- Immunogen A synthetic peptide of human BDNF
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Gene ID
627
12064
24225
-
Swiss Prot
P23560
P21237
P23363
- Synonyms ANON2; BULN2
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
-
Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:500
ICC/IF: 1:200-1:500
FC: 1:20 - Calculated MW 15 kDa
- Observed MW Precursor 28 kDa - Mature 14 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13685
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling BDNF with db15513.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15513 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Western blot analysis of extracts from C6, SH-SY5Y cells using db15513 at 1:1000.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
