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LC3B (DGR11871) Rabbit mAb
db15555
Datasheet
- Background The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human LC3B
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Gene ID
81631
67443
64862
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Swiss Prot
Q9GZQ8
Q9CQV6
Q62625
- Synonyms LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
- Reactivity Human, Mouse, Rat, Pig
- Application WB, IHC, ICC/IF
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Recommended dilution
WB: 1:1000-1:5000
IHC: 1:500-1:2000
ICC/IF: 1:1000 - Calculated MW 15 kDa
- Observed MW 14,16 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11871
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from U-87 MG cells using db15555 at 1:1000. -
Western blot analysis of extracts from MCF-7 cells and Rat muscle tissue using db15555 at 1:4000. -
Western blot analysis of extracts from U-87 MG，PC-12 cells using db15555 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling LC3B with db15555.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15555 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
