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Conjugation/Formulation：Un-conjugated
- Background This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
- Immunogen Recombinant protein of human METTL3
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Gene ID
56339
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Swiss Prot
Q86U44
- Synonyms M6A; IME4; Spo8; MT-A70; hMETTL3
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC: 1:200-1:500
ICC/IF: 1:200-1:500
FC: 1:20
IP: 1:20 - Calculated MW 64 kDa
- Observed MW 64 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11429
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of NIH/3T3 cells labelling METTL3 with db15992.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15992(1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Western blot analysis of extracts from Raji,PC-12,F9 cells using db15992 at 1:1000.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
