Goat Anti Rabbit IgG (H+L)-AF647 - diagbio | 戴格生物

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Goat Anti Rabbit IgG (H+L)-AF647

db10006

Datasheet

Product Name : Goat Anti Rabbit IgG (H+L)-AF647

Cat.No.: db10006

Application : ICC/IF, IHC-F, IHC-P, FC, ELISA

Reactivity : Rabbit

Host species : Goat

Background Secondary antibodies providesignal detection and amplification along with extending the utility of anantibody through conjugation to proteins. Secondary antibodies bind to primaryantibodies, which are directly bound to the target antigen(s).
Immunogen Rabbit IgG
Reactivity Rabbit
Application ICC/IF, IHC-F, IHC-P, FC, ELISA
Recommended dilution ICC/IF: 1:200-1:1000
IHC-P: 1:200-1:1000
IHC-F: 1:200-1:1000
FC: 1:200-1:1000
ELISA: 1:2000-1:20000
Host species Goat
Clonality Polyclonal
Isotype IgG
Purity Affinity Purification
Conjugation AF647
Storage/Stability Store at -20°C. Supplied in PBS, 50% Glycerol, 0.1% BSA. Stable for 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells labelling Cytokeratin 8 with db16057.

The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16057 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF647 (db10006, shown in red). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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