RhoA (DGR11187) Rabbit mAb (PBS Only)
Product Name :
RhoA (DGR11187) Rabbit mAb (PBS Only)
Cat.No.:
db16061-PBS
Synonyms :
ARHA; ARH12; RHO12; RHOH12
Reactivity :
Human, Mouse, Rat
Host species :
Rabbit
Conjugation/Formulation:PBS Only
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Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
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Immunogen
A synthetic peptide of human RhoA
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Gene ID
387
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Swiss Prot
P61586
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Synonyms
ARHA; ARH12; RHO12; RHOH12
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Reactivity
Human,Mouse,Rat
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Application
WB, ICC/IF, FC
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Calculated MW
22 kDa
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Observed MW
22 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Clonality No.
DGR11187
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Concentration
1 mg/mL
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Formulation
PBS Only
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Storage/Stability
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
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Western blot analysis of extracts from C6, HepG2 cells using
db16061 at 1:1000.
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Western blot analysis of extracts from 3T3 cells using
db16061 at 1:1000.
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Immunofluorescence analysis of Jurkat cells labelling RhoA with
db16061.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with
db16061 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
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Package
Price (RMB)
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10μg
1498
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100μg
4498
- 货期:现货
For research use only. Not intended for diagnostic.