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Conjugation/Formulation：Un-conjugated
- Background This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human DIAPH1
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Gene ID
1729
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Swiss Prot
O60610
- Synonyms DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1; mDia1; DIAP1
- Reactivity Human, Mouse, Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:50
FC: 1:100 - Calculated MW 141 kDa
- Observed MW 150 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR36090
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from MCF-7 cells using db16325 at 1:1000. -
Immunofluorescence analysis of MCF-7 cells labelling DIAPH1 with db16325.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16325 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
