hnRNP A1 (DGR36132) Rabbit mAb (PBS Only) - diagbio | 戴格生物

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Recombinant DGRmAb^®

hnRNP A1 (DGR36132) Rabbit mAb (PBS Only)

db16367-PBS

Datasheet

Product Name : hnRNP A1 (DGR36132) Rabbit mAb (PBS Only)

Cat.No.: db16367-PBS

Synonyms : MPD3; UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]
Immunogen A synthetic peptide of human hnRNP A1
Gene ID 3178
15382
29578
Swiss Prot P09651
P49312
P04256
Synonyms MPD3; UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Reactivity Human, Mouse, Rat
Application WB, IHC-P, ICC/IF, FC
Recommended dilution WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:200
FC: 1:100
Calculated MW 39 kDa
Observed MW 36 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR36132
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Concentration 1 mg/ml
Formulation PBS Only
Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.

Western blot analysis of extracts from Jurkat, HepG2 cells and Rat spleen tissue using db16367 at 1:1000.
Immunofluorescence analysis of MCF-7 cells labelling hnRNP A1 with db16367.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16367 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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