您当前的位置：
Prohibitin (DGR34304) Rabbit mAb (PBS Only)
db11502-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- Immunogen A synthetic peptide of human Prohibitin
-
Gene ID
5245
-
Swiss Prot
P35232
- Synonyms PHB1; HEL-215; HEL-S-54e
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
- Calculated MW 30 kDa
- Observed MW 30 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR34304
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from A549, HL-60, C6 cells using db11502 at 1:1000. -
Western blot analysis of extracts from HeLa, HepG2, RAW264.7 cells and Mouse heart tissue using db11502 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using db11502 antibody. -
Immunofluorescent analysis of HeLa cells using db11502 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of A549 cells labelling Prohibitin with db11502.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11502 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
