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Conjugation/Formulation：Un-conjugated
- Background This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- Immunogen A synthetic peptide of human Prohibitin
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Gene ID
5245
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Swiss Prot
P35232
- Synonyms PHB1; HEL-215; HEL-S-54e
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
IP: 1:100 - Calculated MW 30 kDa
- Observed MW 30 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR34304
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from A549, HL-60, C6 cells using db11502 at 1:1000. -
Western blot analysis of extracts from HeLa, HepG2, RAW264.7 cells and Mouse heart tissue using db11502 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using db11502 antibody. -
Immunofluorescent analysis of HeLa cells using db11502 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of A549 cells labelling Prohibitin with db11502.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11502 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
