SMARCA5 (DGR20118) Rabbit mAb (PBS Only)
Product Name :
SMARCA5 (DGR20118) Rabbit mAb (PBS Only)
Cat.No.:
db11595-PBS
Synonyms :
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Reactivity :
Human, Mouse, Rat
Host species :
Rabbit
Conjugation/Formulation:PBS Only
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Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
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Immunogen
A synthetic peptide of human SNF2H
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Gene ID
8467
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Swiss Prot
O60264
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Synonyms
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
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Reactivity
Human,Mouse,Rat
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Application
WB, ICC/IF
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Calculated MW
122 kDa
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Observed MW
122 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Clonality No.
DGR20118
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Concentration
1 mg/mL
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Formulation
PBS Only
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Storage/Stability
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
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Western blot analysis of extracts from K562, C6, 3T3, HeLa cells using
db11595 at 1:1000.
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Immunofluorescent analysis of Jurkat cells using
db11595 antibody (green), and DAPI (blue).
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Immunofluorescence analysis of HeLa cells labelling SMARCA5 with
db11595.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with
db11595 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
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Package
Price (RMB)
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10μg
1898
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100μg
5798
- 货期:现货
For research use only. Not intended for diagnostic.