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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human SNF2H
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Gene ID
8467
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Swiss Prot
O60264
- Synonyms ISWI; SNF2H; hISWI; hSNF2H; WCRF135
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF
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Recommended dilution
WB: 1:1000-1:5000
ICC/IF: 1:100-1:200 - Calculated MW 122 kDa
- Observed MW 122 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR20118
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6, 3T3, HeLa cells using db11595 at 1:1000. -
Immunofluorescent analysis of Jurkat cells using db11595 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling SMARCA5 with db11595.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11595 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
