您当前的位置：
Conjugation/Formulation：Un-conjugated
- Background This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human Caldesmon
-
Gene ID
800
-
Swiss Prot
Q05682
- Synonyms CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
-
Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100
ICC/IF: 1:50
FC: 1:20-1:50
IP: 1:20 - Calculated MW 93 kDa
- Observed MW 70-80 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11987
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from C6, 3T3, HeLa cells using db12512 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human colon cancer using db12512 antibody. -
Immunofluorescence analysis of HeLa cells labelling Caldesmon with db12512.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12512 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
