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Retinoid X Receptor alpha (DGR14758) Rabbit mAb (PBS Only)
db12582-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
- Immunogen A synthetic peptide of human Retinoid X Receptor alpha
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Gene ID
6256
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Swiss Prot
P19793
- Synonyms NR2B1
- Reactivity Human, Mouse, Rat
- Application WB, ICC/IF, IP
- Calculated MW 51 kDa
- Observed MW 51 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14758
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, C6 cells using db12582 at 1:1000. -
Immunofluorescent analysis of MCF-7 cells using db12582 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling Retinoid X Receptor alpha with db12582.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12582 (1:20) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Western blot analysis of extracts from HepG2 cells using db12582 at 1:1000.
订购信息
- Package Price (RMB)
- 10μg 2598
- 100μg 7858
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
