Retinoid X Receptor alpha单克隆抗体(DGR14758) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Retinoid X Receptor alpha (DGR14758) Rabbit mAb

db12582

暑期特惠七夕288

Datasheet

Product Name : Retinoid X Receptor alpha (DGR14758) Rabbit mAb

Cat.No.: db12582

Synonyms : NR2B1

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
Immunogen A synthetic peptide of human Retinoid X Receptor alpha
Gene ID 6256
Swiss Prot P19793
Synonyms NR2B1
Reactivity Human, Mouse, Rat
Application WB, ICC/IF, IP
Recommended dilution WB: 1:1000-1:2000
ICC/IF: 1:20-1:100
IP: 1:20-1:50
Calculated MW 51 kDa
Observed MW 51 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR14758
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot analysis of extracts from HeLa, C6 cells using db12582 at 1:1000.
Immunofluorescent analysis of MCF-7 cells using db12582 antibody (green), and DAPI (blue).
Immunofluorescence analysis of HeLa cells labelling Retinoid X Receptor alpha with db12582.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12582 (1:20) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.
Western blot analysis of extracts from HepG2 cells using db12582 at 1:1000.

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