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Heme Oxygenase 1 (DGR15440) Rabbit mAb (PBS Only)
db14175-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
- Immunogen Recombinant protein of mouse Heme Oxygenase 1
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Gene ID
3162
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Swiss Prot
P09601
- Synonyms HO-1; HSP32; HMOX1D; bK286B10
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 33 kDa
- Observed MW 33 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15440
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from U251, K562, A549 cells using db14175 at 1:500. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db14175 antibody. -
Western blot analysis of extracts from Rat spleen tissue using db14175 at 1:1000. -
Western blot analysis of extracts from Mouse spleen tissue using db14175 at 1:1000. -
Immunofluorescence analysis of HepG2 cells labelling Heme Oxygenase 1 with db14175.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14175 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
