Heme Oxygenase 1单克隆抗体(DGR15440) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Heme Oxygenase 1 (DGR15440) Rabbit mAb

db14175

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Datasheet

Product Name : Heme Oxygenase 1 (DGR15440) Rabbit mAb

Cat.No.: db14175

Synonyms : HO-1; HSP32; HMOX1D; bK286B10

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
Immunogen Recombinant protein of mouse Heme Oxygenase 1
Gene ID 3162
Swiss Prot P09601
Synonyms HO-1; HSP32; HMOX1D; bK286B10
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC-P: 1:1000-1:10000
ICC/IF: 1:100-1:200
FC: 1:20-1:50
IP: 1:20-1:50
Calculated MW 33 kDa
Observed MW 33 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR15440
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot analysis of extracts from U251, K562, A549 cells using db14175 at 1:500.
Immunohistochemical analysis of paraffin-embedded human lung cancer using db14175 antibody.
Western blot analysis of extracts from Rat spleen tissue using db14175 at 1:1000.
Western blot analysis of extracts from Mouse spleen tissue using db14175 at 1:1000.
Immunofluorescence analysis of HepG2 cells labelling Heme Oxygenase 1 with db14175.

The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14175 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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