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Thrombospondin 1 (DGR33394) Rabbit mAb (PBS Only)
db14596-PBS
Datasheet

Product Name : Thrombospondin 1 (DGR33394) Rabbit mAb (PBS Only)

Cat.No.: db14596-PBS

Synonyms : TSP; THBS; TSP1; TSP-1; THBS-1

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]
  • Immunogen Recombinant protein of human Thrombospondin 1
  • Gene ID 7057
  • Swiss Prot P07996
  • Synonyms TSP; THBS; TSP1; TSP-1; THBS-1
  • Reactivity Human,Mouse,Rat
  • Application WB, IHC-P, ICC/IF, FC, IP
  • Calculated MW 130 kDa
  • Observed MW 170 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR33394
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from MCF-7, C2C12 cells using db14596 at 1:1000.
  • Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using db14596 antibody.
  • Immunofluorescence analysis of HepG2 cells labelling Thrombospondin 1 with db14596.

    The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14596 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 1898
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.