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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]
- Immunogen Recombinant protein of human Thrombospondin 1
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Gene ID
7057
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Swiss Prot
P07996
- Synonyms TSP; THBS; TSP1; TSP-1; THBS-1
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:1000-1:5000
ICC/IF: 1:100
FC: 1:50-1:500
IP: 1:20-1:50 - Calculated MW 130 kDa
- Observed MW 170 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR33394
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from MCF-7, C2C12 cells using db14596 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using db14596 antibody. -
Immunofluorescence analysis of HepG2 cells labelling Thrombospondin 1 with db14596.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14596 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
